Neuroprotective Effect of Cannabis Sativa on Stress Induced Biochemical and Histological Changes in the Brain of Wistar Rats

Authors

  • Olalekan Oladimeji Department of Human Anatomy, Faculty of Basic Medical Sciences, College of Medical Sciences, Ahmadu Bello University (A.B.U), Zaria- Nigeria https://orcid.org/0000-0002-3789-4170
  • Uduak Umana Department of Human Anatomy, Faculty of Basic Medical Sciences, College of Medical Sciences, Ahmadu Bello University (A.B.U), Zaria- Nigeria https://orcid.org/0000-0003-2096-7238
  • Abel Agbon Department of Human Anatomy, Faculty of Basic Medical Sciences, College of Medical Sciences, Ahmadu Bello University (A.B.U), Zaria- Nigeria https://orcid.org/0000-0003-1390-8133
  • Serene Daodu Department of Human Anatomy, Faculty of Basic Medical Sciences, College of Medical Sciences, Ahmadu Bello University (A.B.U), Zaria- Nigeria https://orcid.org/0000-0003-4428-0094
  • Aisha Usman Department of Human Anatomy, Faculty of Basic Medical Sciences, College of Medical Sciences, Ahmadu Bello University (A.B.U), Zaria- Nigeria https://orcid.org/0000-0002-9470-023X

DOI:

https://doi.org/10.5281/zenodo.7743964

Keywords:

Antioxidant, Neurodegeneration, Neuroprotective, Oxidative stress

Abstract

Background: Stress continues to be a rising global health concern that can trigger neurodegenerative diseases. Available treatment options focus more on the alleviation of specific symptoms without effort to control/delay the progression of neurodegenerative diseases triggered by stress. Cannabis sativa l. (C. sativa) at moderate doses has potent psychoactive and antioxidative properties. Objectives: This study assessed the neuroprotective effect of a hydroethanolic extract of C. sativa (HCS) on stress induced biochemical changes in the brains of Wistar rats. Methods: Twenty-five (25) Wistar rats were used in the present study and were divided into 5 groups (n=5). Group I (H2O, 1 ml/kg); group II (2 hours of restraint stress); group III (diazepam, (5 mg/kg) + stress; group IV (250 mg/kg C. sativa. + stress); and group V (750 mg/kg of C. sativa + stress). Treatment was via oral route for a period of 21 days. The neuroprotective effect of HCS was evaluated using, biochemical and histological analysis of oxidative stress biomarkers (MDA, SOD, CAT, and GPx) and endogenous enzyme (AChE) activity of the brain of Wistar rats and the CA1 and CA3 regions of the hippocampus of Wistar rats using brain H&E stains, respectively. Results: Biochemical results revealed alterations in the concentration levels of oxidative stress parameters in the hippocampus in stress exposed groups. HCS significantly (p<0.05) ameliorated stress-induced alterations of biochemical parameters. Histoarchitecture alterations were observed in the hippocampus of the stress exposed group, which were mitigated in HCS group in a dose-dependent manner. Conclusion: Findings from this study suggest the efficacy of low dose C. sativa. in ameliorating stress induced biochemical and histological alterations in the brain and could be a potential candidate for application in the management and treatment of stress induced neurodegeneration

Author Biographies

  • Olalekan Oladimeji, Department of Human Anatomy, Faculty of Basic Medical Sciences, College of Medical Sciences, Ahmadu Bello University (A.B.U), Zaria- Nigeria

     

     

     

     

  • Uduak Umana, Department of Human Anatomy, Faculty of Basic Medical Sciences, College of Medical Sciences, Ahmadu Bello University (A.B.U), Zaria- Nigeria

    Professor of Human Anatomy 

    Department of Human Anatomy, Faculty of Basic Medical Sciences, College of Medical Sciences, Ahmadu Bello University (A.B.U), Zaria- Nigeria

     

     

  • Abel Agbon, Department of Human Anatomy, Faculty of Basic Medical Sciences, College of Medical Sciences, Ahmadu Bello University (A.B.U), Zaria- Nigeria

    Human Anatomy (BSc., MSc., PhD)

    Department of Human Anatomy, Faculty of Basic Medical Sciences, College of Medical Sciences, Ahmadu Bello University (A.B.U), Zaria- Nigeria

    Neuroanatomy and Neuroscience Research Unit, Department of Human Anatomy, A.B.U, Zaria

    Microscopy and Stereology Research Unit, Department of Human Anatomy, A.B.U, Zaria

     

     

  • Serene Daodu, Department of Human Anatomy, Faculty of Basic Medical Sciences, College of Medical Sciences, Ahmadu Bello University (A.B.U), Zaria- Nigeria

    Department of Human Anatomy, Faculty of Basic Medical Sciences, College of Medical Sciences, Ahmadu Bello University (A.B.U), Zaria- Nigeria

    Neuroanatomy and Neuroscience Research Unit, Department of Human Anatomy, A.B.U, Zaria

     

     

     

  • Aisha Usman, Department of Human Anatomy, Faculty of Basic Medical Sciences, College of Medical Sciences, Ahmadu Bello University (A.B.U), Zaria- Nigeria

    Department of Human Anatomy, Faculty of Basic Medical Sciences, College of Medical Sciences, Ahmadu Bello University (A.B.U), Zaria- Nigeria

     

     

Published

2023-03-19

How to Cite

1.
Neuroprotective Effect of Cannabis Sativa on Stress Induced Biochemical and Histological Changes in the Brain of Wistar Rats. J Med B Sci Res [Internet]. 2023 Mar. 19 [cited 2024 Nov. 8];4(1-2):71-84. Available from: https://jmbsr.com.ng/index.php/jmbsr/article/view/180

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